ESTIMATION OF PROTEINS BY BRADFORD METHOD

Aim:

To estimate the amount of protein in the given sample by Bradford Assay.

Principle

The protein in solution can be measured quantitatively by different methods. The methods described by Bradford uses a different concept-the protein‘s capacity to bind to a dye, quantitatively. The assay is based on the ability of proteins to bind to coomassie brilliant blue and form a complex whose extinction coefficient is much greater than that of free dye. 

ESTIMATION OF PROTEIN BY LOWRY’S METHOD

Aim:

To estimate the amount of protein in the given sample by Lowry’s method.

Principle:

     The principle behind the Lowry method of determining protein concentrations lies in the reactivity of the peptide nitrogen[s] with the copper [II] ions under alkaline conditions and the subsequent reduction of the Folin- Ciocalteay phosphomolybdic phosphotungstic acid to heteropolymolybdenum blue by the copper-catalyzed oxidation of aromatic acids [Dunn, 13].